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KMID : 0358920110380020129
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Journal of the Korean Academy of Pedodontics
2011 Volume.38 No. 2 p.129 ~ p.136
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THE ANTIMICROBIAL EFFECT OF HORSERADISH (ARMORACIA RUSTICANA) ROOT EXTRACTS AS AN ENDODONTIC IRRIGANT
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Yun Hyo-Jin
Park Ho-Won
Lee Ju-Hyun
Seo Hyun-Woo
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Abstract
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Microorganisms are the main causative factors of pulpal and periapical diseases, therefore successful endodontic treatment is depend on the effective elimination of intracanal bacterial populations. Many studies have been reported antimicrobial effect of Allyl isothiocyanate (AIT) which the principle ingredient of Horseradish (Armoracia rusticana) root extracts. The purposes of this study are to evaluate the antimicrobial effectiveness of Horseradish root extracts against Enterococcus faecalis in root canals of extracted human teeth and compare to sodium hypochlorite (NaOCl). Extracted human mandibular premolar root canals were infected with E. faecalis for 21 days, and then irrigated with Horseradish root extracts, NaOCl solution and saline. After canal irrigation, first samples (S1) were taken. After first sampling, the canals were additionally incubated 7 days, and then second samples (S2) were taken. The samples were inoculated on EHI agar plate to determine the colony forming units (CFU). 1. Mean values of CFU in S1 were 5.815×10³ CFU/ml at Horseradish groups, and 3.465×10³ CFU/ml at NaOCI groups. There was no statistically significant differences (p=0.086). 2. Mean values of CFU in S2 were 3.100×10³ CFU/ml at Horseradish groups, and 5.252×10^5 CFU/ml at NaOCI groups. There was statistically significant difference (p<.05). 3. There was no statistically significant differences (p=0.076) between S1 and S2 at Horseradish groups in the mean values of CFU. However, there was statistically significant differences (p<.05) between S1 and S2 at NaOCI groups in the mean values of CFU.
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KEYWORD
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Horseradish (Armoracia rusticana) root extracts, Allyl isothiocyanate (AlT), Endodontic irrigant, Enterococcus faecalis
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